HPLC Column, Ultra Axis C18, 5um, 4.6mm ID x 150mm Length, 80A. Cogent Brand. 1 EA.

Additional Info:

The 59035‑15P Cogent Ultra Axis C18™ HPLC Column is a high surface area C18 stationary phase, engineered for enhanced retention and selectivity in reversed‑phase chromatography.

This column features a fully double end‑capped C18 bonded phase on high‑purity silica with an 80 Å pore size, making it ideal for USP L1 methods that specifically require 80 Å pore structure.

✅ Widely used as a reliable alternate column in USP methods requiring L1 classification with 80 Å pore size

150 mm Length – High Resolution for Analytical Methods

  • Provides increased resolving power vs shorter columns

  • Improves separation of closely related compounds

  • Increases peak capacity for complex mixtures

  • Ideal for method development and validation workflows

✅ Selected when resolution and reproducibility are critical

4.6 mm ID – Standard Analytical Platform

  • Compatible with standard HPLC systems

  • Ideal for:

    • routine QC analysis

    • method validation and transfer

Typical Flow Rate Range

  • ~0.8–1.5 mL/min

✅ Ensures robust, reproducible analytical performance

5 µm Particle – Balanced Efficiency & Durability

  • Provides:

    • Consistent efficiency for analytical separations

    • Long column lifetime

    • Reliable reproducibility

  • Higher surface area vs standard C18 phases results in:

    • Increased retention

    • Altered selectivity profile

✅ Ideal for method optimization and transfer across systems

80 Å Pore Size – USP L1 Method Compatibility

  • Designed specifically for:

    • USP L1 methods requiring 80 Å pore size

  • Provides:

    • Enhanced interaction with small molecules

    • Improved performance in traditional reversed‑phase applications

✅ Ensures method compliance and reproducibility in regulated workflows

Why Choose Ultra Axis C18™ Columns

High Surface Area Selectivity

  • Provides increased retention and unique selectivity

  • Enhances resolution of hydrophobic compounds

USP Method Flexibility

  • Fully compatible with USP L1 methods

  • Suitable for method transfer and validation

Proven Performance

  • Long‑standing use in analytical laboratories

  • Known for reliability and consistency

✅ Ideal for regulated workflows requiring validated performance

Application Performance Advantages

  • Suitable for:

    • Hydrophobic compounds

    • Neutral analytes

    • Long‑chain fatty acids

  • Provides:

    • Increased retention vs standard C18 phases

    • Enhanced separation of non‑polar analytes

✅ Ideal for reversed‑phase analytical workflows requiring strong retention

System & Sample Considerations

  • Compatible with standard HPLC systems

  • Suitable for routine analytical and QC workflows

Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

  • Prevent column fouling

  • Maintain long‑term performance and reproducibility

Typical Applications

  • USP L1 analytical methods

  • Pharmaceutical QC and validation

  • Hydrophobic compound separations

  • Long‑chain fatty acid analysis

  • Method development and transfer

  • Routine HPLC analysis

Important Usage Notes

  • Designed for standard analytical HPLC systems (4.6 mm ID)

  • Optimized for 80 Å pore size USP L1 methods

  • Ideal for high‑resolution analytical workflows

  • Monitor system pressure based on instrument specifications

 

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